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KMID : 0358320070480080815
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Korean Journal of Urology
2007 Volume.48 No. 8 p.815 ~ p.825
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The Effect of Ring-type NF-kappa B(NF-kB) Decoy Oligodeoxynucleotide on the Kidney for an Experimental Unilateral Ureteral Obstruction in Mice
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Kwon Young-Gun
Lee Young-Guk
Kang Il
Lee Eun-Seock
Park Kwan-Kyu
Kum Yoon-Seup
Kim Kyung-Hyun
Park Jae-Shin
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Abstract
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Purpose: The transcription factor, NF-kB, is important in the coordinated expressions of various pro-inflammatory and adhesion molecules. Our hypothesis is that inhibiting the action of NF-kB using a synthetic decoy oligodeoxynucleotide(ODN) can block the underlying inflammatory response in glomerulonephritis.
Materials and Methods: Forty two male C57BL6 mice, weighting 25g, were divided into four groups; in group 1, 2 mice were used as normal controls; in group 2, a unilateral ureteral obstruction(UUO) was induced in 12 mice; in group 3, 20 UUO mice were treated using ring-type NK-kB decoy ODN; and in group 4, 8 UUO mice were treated using scramble type NF-kB decoy ODN. The mice were killed 1, 3, 5 and 7 days after the NF-kB decoy ODN injections, and the blood urea nitrogen(BUN), and expressions of tumor necrosis factor-?(TNF-?), interleukin-?(IL-?), fibronectin, vascular cell adhesion molecule(VCAM) and monocyte chemotactic protein-1(MCP-1), as well as the histopathological findings, analyzed in each group.
Results: The serum levels of BUN, TNF-?, IL-?, fibronectin, VCAM and MCP-1 were increased in group 2, but decreased in group 3. The histopathological findings of the kidneys in group 3 were most similar to those found in the control(group 1).
Conclusions: NF-kB decoy ODN treatment substantially inhibited the disease, with reductions in the histological damage and the renal expressions of inflammatory cytokines.
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KEYWORD
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NF-kappa B, Transcription factors, Ureteral obstruction
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